Author(s): Sakiko Yaegashi

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Abstract: Environmental DNA is attracting attention as a new species diversity monitoring method. In the case of eDNA metabarcoding for aquatic insects, which is an indicator of anthropogenic disturbance, non-target taxa (i.e., algae, bacteria) are often detected, which consumes the NGS sequence capacity. In this study, we investigated PCR conditions with high detection efficiency for aquatic insects. In the case of the eDNA metabarcoding to highly detect non-target taxa, it is helpful to detect rare taxa by reducing PCR cycles and by increasing PCR replication.

DOI:

Year: 2022