Author(s): Sakiko Yaegashi; Kozo Watanabe

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Keywords: Next-generation sequencing; Habitat fragmentation; Gene flow; River ecosystem; Macroinvertebrate; Amplicon

Abstract: Anthropogenic habitat fragmentations in rivers such as damming may interrupt dispersals of stream invertebrates, reducing gene flow between fragmented populations. A huge number of studies have been conducted to reveal the effect of habitat fragmentation on spatial population genetic structure. However, their foci have been limited to one or a few species rather than multiple co-occurring species in communities due to the limited analytical power of classic DNA sequencing technology (i.e. Sanger sequencing). As an alternative approach, we employed Next-Generation Sequencing (NGS) to reveal the population genetic structures of multiple species in focal communities simultaneously using site-specific parallel DNA tag. NGS is an emerging new technology to read huge number of DNA sequences in parallel and in one analysis, which highly contribute to save time and cost. In this study, we examined the spatial population genetic structures of multispecies of steam insects under potential influences of habitat fragmentation in rivers (e.g., dams) using NGS. The Cytochrome Oxidase I (COI) region was amplified from pooled template DNA. The PCR products from each site with site-specific tags were mixed, and sequenced together at once using Mi Seq (Illumina). Total sequenced reads were11.6M reads. The overall presentation discusses the ecological advantage of SBT on freshwater insects interrupted by dam.

DOI:

Year: 2016